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Title: which of the following is NOT TRUE with regard to replication cycles used in Polymerase Chain Reaction? Post by: FIREMEDIC73 on Oct 16, 2012 HERE 'C' STANDS FOR CELSIUS
A. 90-96C - DNA is denatured into single strands B. 50-60C - The primers anneal to their complementary sequences C. 72C - The polymerase binds and extends a complementary DNA strand from each primer D. 80C - The polymerase adds dNTp's from 3'- 5, reading the template from 5'- 3' Title: which of the following is NOT TRUE with regard to replication cycles used in Polymerase Chain Reaction? Post by: sms305 on Oct 16, 2012 D.
The polymerase has to attach the dNTP to a free OH group at the 3' end. This means that the dNTP's are added in a 5'> 3' direction. |