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Title: If you have 500ml of a 10x buffer stock, how much concentrated buffer and water wuld you need to use? Post by: bugmenot1 on Oct 19, 2012 You have 500ml of a 10x buffer stock. You need to make 500ml of 1x buffer to use for an experiment. How much concentrated buffer and water would you need to use?
Ahh, I keep getting like two different answers for this one. Title: If you have 500ml of a 10x buffer stock, how much concentrated buffer and water wuld you need to use? Post by: BuggerFormic on Oct 19, 2012 Content hidden
Title: If you have 500ml of a 10x buffer stock, how much concentrated buffer and water wuld you need to use? Post by: bugnsprout on Oct 19, 2012 Imagine that you needed a litre 1x from 10x. 100 mls 10x + 900 mls water would give you the required dilution but this would be twice as much as you need so half the amounts: 50 mls 10x + 450 mls water gives you what you need.
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