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Title: We tested a new restriction enzyme on a plasmid of 6.3kb. The digest was run in an agarose gel ... Post by: allan2009 on Oct 13, 2010 We tested a new restriction enzyme on a plasmid of 6.3kb. The digest was run in an agarose gel against linear standards. But only a single band was present in the plasmid digest lane and when its size was calculated from the standard curve, it was found to be only about 2kb. (excluding experimental error) i am not usre why?
Title: We tested a new restriction enzyme on a plasmid of 6.3kb. The digest was run in an agarose gel ... Post by: bio_man on Oct 13, 2010 I'm assuming that the restriction enzyme must have "cut" the plasmid in 3 different areas evenly spread throughout the genome (i.e. 6.3 divided by 3 = 2.1 kb).
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