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Laboratory Help Upper-Year Courses Topic started by: rixa85 on Dec 25, 2012



Title: do keratinocytes contain collagen and fibronectin?
Post by: rixa85 on Dec 25, 2012
I am doing an ELISA with keratinocytes. I want to measure collagen and fibronectin. However, I need to know if these keratinocytes make these things to begin with. :)


Title: do keratinocytes contain collagen and fibronectin?
Post by: tonigra on Dec 25, 2012
No Keratinocytes is epidermis, not dermis or connective tissue, so it does not contain collagen.

As for fibronectin  - "Cellular fibronectin is assembled into the extracellular matrix, an insoluble network that separates and supports the organs and tissues of an organism. Fibronectin plays a crucial role in wound healing.[6] Along with fibrin, plasma fibronectin is deposited at the site of injury, forming a blood clot that stops bleeding and protects the underlying tissue."

I do not believe keratinocyte contain collage or fibronectin