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Title: What marker molecules should one use in order to identify cell organelles? Post by: rivers.butterfly on Mar 5, 2013 For example we could use succinate dehydrogenase as a mitochondrial marker enzyme, after doing density gradient centrifugation. What other similar enzymes or molecules should we use in order to identify other cell organelles? Thank you!
Title: What marker molecules should one use in order to identify cell organelles? Post by: michelle.muhs on Mar 5, 2013 you could use antibodies to do this. Find a protein that localizes to a speciffic organelle, such as histones for the nucleus, a membrane receptor for the cell membrane, COX2 for the ER ect. Then do a western blot on your extract.
Title: What marker molecules should one use in order to identify cell organelles? Post by: fireheart_gir on Mar 5, 2013 Content hidden
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