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Title: Expressing two different membrane proteins on the same promoter? Post by: anonyvous on Jul 25, 2017 How could I generate a stable mammalian cell line that expresses two different membrane proteins on the same promoter? I want to know what strategies I could take.
Title: Re: Expressing two different membrane proteins on the same promoter? Post by: dtimmons95 on Jul 26, 2017 Since you wish to express two gene under the same promoter, you can use an IRES sequence, or other such elements, between the genes to create a bicistronic gene. Alternatively, you can use a vector containing a bidirectional promoter, such as the pBI backbone. In this case you clone each gene into the backbone separately.
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