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Title: Creation of CFTR Knockout Embryonic Stem Cells by Positive-Negative Selection Post by: willuhelp on Jul 23, 2013 I am having trouble understanding what is going on here. What I got thus far is that the more preferred fate is Homologous recombination (targeted approach). Next, you basically plate the ES cells that are both neomycin & ganciclovir resistant (homologous recombination). I basically do not know if I am on the right track. Any feedback of any sort will help.
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