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Title: Electrophoresis + Western Blotting Post by: micro on Nov 20, 2013 When studying the proteome of an organism / microbe very often protein extract is electrophoresed and then western blotting is performed with specific antibodies to a particular protein of interest. If the identity of a protein band after electrophoresis is not known (i.e. the actual protein is not known), how would you quantify the volume if you cannot perform western blot...since you don't know what protein it is and hence don't know what antibodies to use?
Title: Re: Electrophoresis + Western Blotting Post by: padre on Nov 21, 2013 HI micro
I would use a more concentrated antibody if there is not enough protein. Not sure I'm understanding this. Title: Re: Electrophoresis + Western Blotting Post by: micro on Nov 21, 2013 Don't think you understood me. My question is that if you don't know what the proteins are, how do you measure their quantity by antibodies after western blotting? To use antibodies, you need to know what your antigen (in this case protein) is.
Title: Re: Electrophoresis + Western Blotting Post by: padre on Nov 21, 2013 Interesting question. Wouldn't there be an infinite number of antigens possible?
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