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Title: Struggling with Vector Project - Help!!!? Post by: |R2r| on Jan 21, 2014 Hi,
Im doing a really annoying in silico cloning project (using vector NTI software). DNA and protein technology isnt really my forte (Im rubbish at it) and I was wondering if someone who knows about these things could tell me if Im in the right direction. Ok so I have to: Design and construct an expression vector based on pGEX-2T that could be used to express my target protein - FAS2 (arabidopsis thaliana fasciata 2) as a recombinant fusion protein with glutathione S transferase in a bacterial host Okay now I'm sorry if this is a REALLY stupid question, but my stupid Uni has pretty much piled this assignment on us without any actual teaching so does this mean that im going to use pGEX-2T as my vector.......and fuse my FAS2 protein with glutathione S transferase. so stick that fusion protein into pGEX-2T - and then stick the entire thing in a bacterial host??? Just want to know if Im totally lost and retarded etc. Thanks for any help anyone can give me!!!! Cheers Title: Struggling with Vector Project - Help!!!? Post by: miaplayer93 on Jan 21, 2014 Content hidden
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