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Title: Measuring Gel's Distance and Fragment Size Post by: beii530 on Mar 2, 2014 I have a picture that looks like this:
(http://depts.washington.edu/soslab/mw/images/a/a2/Lab3pic_labeled.JPG) And I have this information: (http://www.thermoscientificbio.com/uploadedImages/Products/DNA_Electrophoresis/DNA_Ladders/sm010_fam.jpg) I also have this Lambda DNA Restriction Map, but I don't really know how to use it: (http://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/PublishingImages/act3fig08.jpg) So I was confused on how to measure the marker lane of distance traveled, & DNA fragment size? I'm a bit lost since I can't really tell whether if measuring on this picture would be accurate compared to the smaller version I have on an acetate sheet where it accurately has the spots where each bands were marked. But it's looks a bit confusing, so I'm using this ideal gel to measure. Or it wouldn't matter at all which version I have? Anyway, is there a specific way to measure the distance traveled and the DNA fragment size? I've been having a tough time searching it on Google since I'm not sure if I'm using the correct key words. Oh oops nevermind. I think I might have figured it out. I use the second link I posted to estimate the bp of each band right? And for my teacher, wants it in centimeters. So I would just use a ruler to measure from each well to the leading edge of each band right? Sorry about the post then. Unless I'm wrong. Anyway, thanks. Title: Re: Measuring Gel's Distance and Fragment Size Post by: padre on Mar 3, 2014 Sorry for the late reply.
And for my teacher, wants it in centimeters. So I would just use a ruler to measure from each well to the leading edge of each band right? Yes. |