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Title: Describe the Sanger method of DNA sequencing. What will be an ideal response Post by: Mirbek on Jan 22, 2018 Describe the Sanger method of DNA sequencing.
What will be an ideal response Title: Describe the Sanger method of DNA sequencing. What will be an ideal response Post by: PinBoi on Jan 22, 2018 Complete DNA replication will occur when normal deoxynucleotides (dNTP) are used. When dideoxynucleotides
are incorporated into the growing chain (ddNTP), the chain terminates replication because the hydroxyl group is missing that is necessary for the next nucleotide to be added. If DNA replication occurs in a mixture composed mostly of normal dNTPs but also a small amount of ddNTPs, then DNA chains of different lengths will be produced. If one wishes to sequence a particular piece of DNA, then it is aliquoted into four parts. A primer is added to each aliquot because DNA polymerase needs a primer to start replication, and this insures that DNA replication begins at the same point in each piece of DNA. In one part, replication occurs with normal dNTPs plus a small amount of dideoxyadenine (ddATP); in the next, dNTPs plus dideoxythymine (ddTTP) followed by dideoxyguanine (ddGTP) and dideoxycytosine (ddCTP). When the DNA fragments are analyzed from each aliquot, the point at which any particular chain terminated corresponds to the dideoxynucleotide added to that aliquot. When the fragments are analyzed together using high resolution denaturing polyacrylamide gel electrophoresis, the sequence of the original fragment can be determined. Title: Describe the Sanger method of DNA sequencing. What will be an ideal response Post by: Mirbek on Jan 22, 2018 Thank you for being such a great leader
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