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Title: The culture may be getting too old. When bacteria die their cell walls degrade a Post by: leminhkhai on Jun 22, 2014 The culture may be getting too old. When bacteria die their cell walls degrade and some may not retain the primary stain. The bacteria that haven't completely degraded may retain the primary stain. Therefore you would get red and purple cocci. Gram stains should be done in less than 24 hours of culture.
Thank you! Title: Re: The culture may be getting too old. When bacteria die their cell walls degrade a Post by: bio_man on Jun 22, 2014 Sorry, what's the question?
Title: Re: The culture may be getting too old. When bacteria die their cell walls degrade a Post by: leminhkhai on Jun 22, 2014 Suppose you are viewing a sample from a PURE culture and
observe both red and purple cocci. What are the possible problems that would cause such an inconclusive result? |