Title: You are given four additional polymeric materials (A, B, C, D) to examine for potential as a ...
Post by: raquelwoods14 on Jan 29, 2018
You are given four additional polymeric materials (A, B, C, D) to examine for potential as a
vascular graft material, and are told to use size-exclusion chromatography to determine the
approximate molecular weights of the unknown polymers. You obtain the following
information for the first three samples (A, B, C).
a. Assuming monodisperse samples, what is the molecular weight of each?
Standard 1: Molecular weight 50,000 g/mol
Standard 2: Molecular weight 40,000 g/mol
Standard 3: Molecular weight 35,000 g/mol
Standard 4: Molecular weight 20,000 g/mol
Standard 5: Molecular weight 10,000 g/mol
Unknown A: Peak amount polymer eluted at 7.4 min
Unknown B: Peak amount polymer eluted at 8.4 min
Unknown C: Peak amount polymer eluted at 9.6 min
b. A problem commonly encountered in SEC is overloading the chromatography column by
injection of a highly concentrated sample, which results in broadening of the peak on the
chromatogram. You injected a highly concentrated sample of unknown D into the
chromatography column. Which of the following curves would result? Pick X or Y and
describe why, based on molecular interactions of the sample with the column.
vascular graft material, and are told to use size-exclusion chromatography to determine the
approximate molecular weights of the unknown polymers. You obtain the following
information for the first three samples (A, B, C).
a. Assuming monodisperse samples, what is the molecular weight of each?
Standard 1: Molecular weight 50,000 g/mol
Standard 2: Molecular weight 40,000 g/mol
Standard 3: Molecular weight 35,000 g/mol
Standard 4: Molecular weight 20,000 g/mol
Standard 5: Molecular weight 10,000 g/mol
Unknown A: Peak amount polymer eluted at 7.4 min
Unknown B: Peak amount polymer eluted at 8.4 min
Unknown C: Peak amount polymer eluted at 9.6 min
b. A problem commonly encountered in SEC is overloading the chromatography column by
injection of a highly concentrated sample, which results in broadening of the peak on the
chromatogram. You injected a highly concentrated sample of unknown D into the
chromatography column. Which of the following curves would result? Pick X or Y and
describe why, based on molecular interactions of the sample with the column.
Title: You are given four additional polymeric materials (A, B, C, D) to examine for potential as a ...
Post by: qdolet on Jan 29, 2018
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