|
Title: Trans-acting or cis-acting mutation help. Post by: Scubastevie00 on Jul 30, 2014 My laboratory has identified a new OPERON in E. coli—the Jun operon. We have identified six genes in the operon: JunA, JunB, JunC, JunD, JunE & JunF. We have identified one mutation in the operon: JunA-. Describe the experiment you would do to determine whether JunA- is a Trans-acting Factor or a Cis-acting element?
Title: Re: Trans-acting or cis-acting mutation help. Post by: skip5284 on Aug 4, 2014 PaJaMo experiment.
Constructing Partially Diploid cells in which the section of the chromosome from a wild type cell carrying the Jun genes was intorduced into cell carrying mutant version of Jun genes. Now when the chromosomal genes are constitutively expressed, In case of trans-acting factor : Because of a mutation in JunA gene, the wild type copy restores the actual function. In case of cis-acting element : mutation causing constitutive expression in Jun operator, it could not be complemented by trans factor. The operator functions only in cis. |