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Biology-Related Homework Help Cell Biology Topic started by: Paolaemy on Feb 3, 2018



Title: One strand of a section of DNA isolated from the bacterium E. coli reads: 5-GTAGCCTACCCATAGG-3 ...
Post by: Paolaemy on Feb 3, 2018
One strand of a section of DNA isolated from the bacterium E. coli reads:
 
  5-GTAGCCTACCCATAGG-3
 
  A. Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will be the sequence of the mRNA in this region (make sure you label the 5 and 3 ends of the mRNA)?
  B. How many different peptides could potentially be made from this sequence of RNA, assuming that translation initiates upstream of this sequence?
  C. What are these peptides? (Give your answer using the one-letter amino acid code.)


Title: One strand of a section of DNA isolated from the bacterium E. coli reads: 5-GTAGCCTACCCATAGG-3 ...
Post by: ally on Feb 3, 2018
A. 5-GUAGCCUACCCAUAGG-3
B. Two. (There are three potential reading frames for each RNA. In this case, they are
GUA GCC UAC CCA UAG
UAG CCU ACC CAU AGG
AGC CUA CCC AUA GG?  
The center one cannot be used in this case, because UAG is a stop codon.)
C. VAYP
SLPIG
Note: PTHR will not be a peptide because it is preceded by a stop codon.


Title: One strand of a section of DNA isolated from the bacterium E. coli reads: 5-GTAGCCTACCCATAGG-3 ...
Post by: Paolaemy on Feb 3, 2018
Thank you so very much, was really helpful and kind of you to answer