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Biology-Related Homework Help Cell Biology Topic started by: ehd123 on Oct 23, 2014



Title: Side scatter (flow cytometry)
Post by: ehd123 on Oct 23, 2014
Are fibroblasts considered granular cells?

What about red blood cells versus white blood cells? Are all hematopoietic cells granular?


Title: Re: Side scatter (flow cytometry)
Post by: Alexx on Oct 23, 2014
You probably mean a cell with granules?
Is this what you have in mind? : http://en.wikipedia.org/wiki/Granulocyte

If you specifically meant "granular cells" then I can't find what it is except this (which is highly unlikely): http://en.wikipedia.org/wiki/Granule_cell


Title: Re: Side scatter (flow cytometry)
Post by: ehd123 on Oct 23, 2014
I meant granulocytes..

If a side scatter of flow cytometry was too dark, does that mean that the cell is granular? Because a side scatter is a measure of granularity?


Title: Re: Side scatter (flow cytometry)
Post by: Alexx on Oct 23, 2014
Well, granulocytes is a group of cells that contains the related white blood cells.

Now, about granules, I think they are present at many cells (for example as lysosomes or secretory vesicles), it's just that, their density is high at granulocytes.

Did you observe something unexpected?


Title: Re: Side scatter (flow cytometry)
Post by: ehd123 on Oct 23, 2014
Hmm no not really, but while I was rehearsing my presentation, I noticed that the majority of the flow cytometry results were side scatter measuring the granulocity of the cells instead of the number of the cells.. And  I don't really get why they were measuring the granulocity..
Is it perhaps because they were making sure that the cells were transitioning from fibroblasts to white blood cells? Hence more granulocity?


Title: Re: Side scatter (flow cytometry)
Post by: Alexx on Oct 23, 2014
Hm, possibly but I don't know... I just wanted to add that, doesn't each dot on the graphs represent an individual cell? If it's like that, then it doesn't matter how much dark the graph is, but where the dots are located.


Not that this will explain anything though....... Since I don't really know why the flow cytometry had to be used anyways.