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Title: How many polypeptide chains are in this protein? Post by: karagolian on Oct 25, 2014 An oligomeric protein has a molecular weight of 415000. A 740 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger\'s reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 1.8 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the alpha-amino groups of other amino acids could be found
How many polypeptide chains are in this protein? (Enter your answer as a whole number). Title: Re: How many polypeptide chains are in this protein? Post by: hooglandm on Oct 25, 2014 Calculate the mw of the protein that was provided. From there, you divide 1.8 so it will 1.8/mw of protein...then you divide 740mg/415000mmol= 0.0018...then you divide again the values that you got from your protein and enter the answer as a whole #. for example: you calculated 3.4 for your final answer you will only have to put 3.
Title: Re: How many polypeptide chains are in this protein? Post by: karagolian on Oct 25, 2014 I saw this all over the Internet, I don't understand it. Would appreciate if you solve it with the numbers given. Thanks.
Title: Re: How many polypeptide chains are in this protein? Post by: hooglandm on Oct 25, 2014 Content hidden
Title: Re: How many polypeptide chains are in this protein? Post by: karagolian on Oct 25, 2014 This really helped a lot! I solved it, I had a similar compound with 2 CH2 and a S attached to the one you showed, Thanks So much!!!
Title: Re: How many polypeptide chains are in this protein? Post by: brendan morrow on Sep 3, 2020 thank you
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