Biology Forums - Study Force

Part B - Inserting the vgp gene into a cloning vector
In the second step of the cloning procedure, DNA fragments produced by a restriction enzyme are inserted into a cloning vector that has been cut by the same enzyme. The vector used in this tutorial is a plasmid.

To clone the vgp gene into the plasmid below, a restriction enzyme must do all of the following:

    cut the human DNA on both sides of the vgp gene, but not inside the gene
    cut the plasmid without cutting inside the ampicillin resistance gene (ampR ), shown in blue (The importance of the ampR gene will be explained in Part C.)
    produce sticky ends when it cuts both the human DNA and the plasmid, so that the human DNA fragments can combine with the plasmid

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