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Title: Using a luciferase reporter system in tissue culture cells, researchers from the University of Calif Post by: daPatriot on Apr 7, 2018 Using a luciferase reporter system in tissue culture cells, researchers from the University of California, San Francisco found that ethanol stimulates transcription of genes in brain cells possibly involved in adaptive responses to alcohol. This process was found to depend on PKA activation. By analogy to other PKA dependent transcription activation pathways, describe a possible pathway for this transcription induction. What other proteins would be involved?
Title: Re: Using a luciferase reporter system in tissue culture cells, researchers from the University of ... Post by: bio_man on Apr 7, 2018 All genes regulated by PKA contain a cis-acting DNA sequence called the CRE (cAMP response element.) Activated PKA phosphorylates the CREB protein. Together with the co-activator, CBP/300, phosphorylated CREB protein can bind to CRE sequences of target genes to activate transcription.
Reference for the research cited in this question: Asher, O., et al. 1} Ethanol stimulates cAMP-responsive element (CRE)-mediated transcription via CRE-binding protein and cAMP-dependent protein kinase, J. Pharmacol Exp Ther. 301(1): 66–70. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11907158&dopt=Abstract |