Title: Interpret the data for this myosin experiment? Post by: bottledwater92 on Mar 13, 2020 Hello, I'm stuck on a question, specifically ii. Could anyone help me out? Also, for i I've put the empty vector is the control, is this correct?
In the first set of experiments, COS-7 cells were manipulated (transfected) to Introduce a plasmid vector containing the coding sequence for myosin 'IA or myosin 118 so that they could overexpress the isoform in question (i.e. to increase the level of expression beyond the level of endogenous expression of the gene). Note that COS-7 cells transfected with myosin 'IA or myosin 118 not only express the transfected isoform but also express their endogenous myosin Il isoforms and therefore contain more myosin Il than non-transfected cells. The researchers seeded a known number of COS-7 cells in a cell culture dish and transfected cultures to overexpress either myosin IA or myosin 118, as described above. After three days, they stained the nuclei of the cells with a DNA-binding dye and captured images of the cultures using a fluorescence microscope (Figure 1 By counting the nuclei within a fixed field-of-view it is possible to determine the number of cells that have adhered to the culture dish in the different cultures, as only the nuclei of adherent cells will be visible. a. The following part-questions require you to analyse and interpret the data presented in Figure 1. i. In Figure 1, which of the treatments is the control for this particular experiment? Explain your answer. (2 marks) ii. For each of the three different cultures in Figure 1 , count and record how many cells have adhered to the culture dishes per field and then, for each of the experimental treatments, express the number of adherent cells as a % of those in the control. (3 marks) Title: Re: Interpret the data for this myosin experiment? Post by: bio_man on Mar 13, 2020 Content hidden
Title: Re: Interpret the data for this myosin experiment? Post by: bottledwater92 on Mar 13, 2020 Ah ok I was interpreting the question wrong, I was dividing the cells in the empty vector by the other 2 and kept getting odd percentages. Thank you for the help, all the other questions relate to drawing a graph based on the percentages which I now can do.
Title: Re: Interpret the data for this myosin experiment? Post by: bottledwater92 on Mar 13, 2020 I counted 85 for my IIB, divided by 12 = 7.08 x 100 to get a percentage but it gives me 708%?
Title: Re: Interpret the data for this myosin experiment? Post by: bio_man on Mar 13, 2020 The math is right, no doubt about that.
If what I said is correct, then report that number. I'm not 100% sure that's what you do, but it makes sense. The ratio is definitely around 7 to 1. You could also say that the control colony consists of 12/85 * 100%, but I like the former way. Title: Re: Interpret the data for this myosin experiment? Post by: bottledwater92 on Mar 14, 2020 I see, I have to plot a graph, so would it be stupid to say a 708% increase from the empty vector? I'm having a hard time interpreting the question.
Title: Re: Interpret the data for this myosin experiment? Post by: bio_man on Mar 14, 2020 Yes, 708% increase is right. That's precisely what we're witnessing
Title: Re: Interpret the data for this myosin experiment? Post by: bottledwater92 on Mar 15, 2020 brilliant, thanks!
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