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Title: Does the volume of substrate affect enzyme activity? Post by: i_love_science on Dec 31, 2021 I know that the substrate concentration affects enzyme activity, but does the volume of substrate affect enzyme activity?
For an example situation, beaker 1 has 5 enzymes and 10 substrate molecules in 10 mL. If the volume of substrate is increased in beaker 2, which still has 5 enzymes, but now has 20 substrate molecules in 20 mL (same concentration, x2 volume), does the enzyme activity change? Thanks! Title: Re: Does the volume of substrate affect enzyme activity? Post by: Laura0624 on Dec 31, 2021 I'd assume so. Specific enzyme activity (usually stated simply as 'specific activity') is the number of enzyme units per ml divided by the concentration of substrate/protein in mg/ml. Specific activity values are therefore quoted as units/mg or nmol/min/mg (if unit definition B is applied).
Title: Re: Does the volume of substrate affect enzyme activity? Post by: Semantha Lorez on Aug 6, 2022 Enzymes will work best if there is plenty of substrate. As the concentration of the substrate increases, so does the rate of enzyme activity.
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