Title: Jacob and colleagues developed a time-of-entry mapping technique. They used the genes for threonine ...
Post by: vanessa_vela on Jan 4, 2022
Jacob and colleagues developed a time-of-entry mapping technique. They used the genes for threonine and leucine synthesis (thr and leu), which are near the oriT site, as selected markers. Additionally, they used an E. coli F- strain P678 that has dysfunctional variants for all of the genes studied. They also used an HfrH strain that carried a functional allele for all genes studied. Which of the following statements is FALSE regarding the time-of-entry profile they acquired?
▸ Had the experiment continued, a time of entry map could be viewed for the entire E. coli chromosome.
▸ The azi gene is closer to the thr and leu genes than the ton, lac, and gal genes.
▸ The ton gene is found between the azi and lac genes.
▸ The lac+ allele appears at about 16 or 17 minutes.
▸ At 40 minutes, nearly 20% of exconjugants screened for the selected markers have the gal+ allele.
▸ Had the experiment continued, a time of entry map could be viewed for the entire E. coli chromosome.
▸ The azi gene is closer to the thr and leu genes than the ton, lac, and gal genes.
▸ The ton gene is found between the azi and lac genes.
▸ The lac+ allele appears at about 16 or 17 minutes.
▸ At 40 minutes, nearly 20% of exconjugants screened for the selected markers have the gal+ allele.
Title: Jacob and colleagues developed a time-of-entry mapping technique. They used the genes for threonine ...
Post by: PHchick on Jan 4, 2022
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