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Biology-Related Homework Help Genetics and Developmental Biology Topic started by: mmora2 on Jan 4, 2022



Title: Why are next generation DNA sequencing technologies known as sequencing-by-synthesis?
Post by: mmora2 on Jan 4, 2022
Why are next generation DNA sequencing technologies known as sequencing-by-synthesis?

▸ The sequencing occurs during S phase of the cell cycle.

▸ RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined.

▸ Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated.

▸ Incorporated nucleotides are determined while they are being added to a growing DNA strand.

▸ The complete DNA strands are synthesized then sequenced.


Title: Why are next generation DNA sequencing technologies known as sequencing-by-synthesis?
Post by: smithan00000 on Jan 4, 2022
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