Biology Forums - Study Force

Which of the following lists the steps for cDNA library preparation in the correct order?

I.	Partially degrade the mRNA with RNase H

II.	Isolate mRNA from a specific cell/ tissue with an oligo-dT primer that hybridizes to poly-A tail

III.	Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers

IV.	Ligate cDNAs into a cloning vector

V.	Create a single-stranded DNA molecule complementary to the mRNA using reverse

transcriptase


▸ I, II, III, IV, V

▸ II, V, I, III, IV

▸ II, III, V, I, IV

▸ V, III, I, IV, II

▸ II, V, IV, III, I

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