Biology Forums - Study Force

In which order should the following steps be done to identify a quantitative trait locus (QTL) gene that contributes to the phenotypic variation in a trait?

I.	DNA and protein sequence analyses are used to determine if there is a correlation with

phenotypic variation

II.	Each of the offspring is individually backcrossed with the same parent, usually the one

expressing the phenotype more strongly

III.	Lod scores are calculated for each genetic marker to find QTLs

IV.	The genotype of the progeny at genetic markers located every 5-10 cM is determined for each

introgression line

V.	Two individuals which exhibit the extremes of a specific phenotype are crossed

VI.	DNA sequence variants among introgression lines are analyzed to find candidate genes

▸ V, II, IV, III, VI, I

▸ I, V, III, II, IV, VI

▸ V, IV, II, III, I, VI

▸ I, II, IV, III, VI, V

▸ V, IV, III, II, VI, I

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