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Title: Biosynthetic Cocaine Plasmid construction Post by: Izzy76 on Aug 9, 2022 Hey there,
I am currently working on constructing a plasmid used for biosynthetic cocaine and want some reviews on it to spot some mistakes i may have made. I overexpressed all off the precursors and added a histidine tag for purification. All the genes are from uniprot. Also i want to know which genes to knockout to improve Flux. Here is the Link https: //benchling.com/s/seq-tW7Olg2xpVhawgY4FqyI?m=slm-7auYvm6cfA9merXCuLL1 Help much appreciated Izzy Title: Re: Biosynthetic Cocaine Plasmid construction Post by: bio_man on Aug 10, 2022 Hi Izzy76 (https://biology-forums.com/index.php?action=profile;u=1169438)
You lost me at "plasmid used for biosynthetic cocaine". You're planning on inserting this plasmid into a species to produce synthetic cocaine. Is this a novel procedure that you invented? Where can I read up on it? Title: Re: Biosynthetic Cocaine Plasmid construction Post by: Izzy76 on Aug 14, 2022 It is a novel procedure i cam up with. Did some research about similiar molecules like tropine and made this plasmid as result
Title: Re: Biosynthetic Cocaine Plasmid construction Post by: Izzy76 on Oct 15, 2022 Can you please delete this post as this serves no longer purpose for me
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