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Title: Explain the relationship between solute concentration and osmotic pressure. Post by: amv1502 on Mar 19, 2011 This is Physioex lab 1 Activity 3, any help would be great
Do you see any evidence of pressure changes in either beaker, using any of the four membranes? If so, which membrane(s)? Does NaCl appear in the right beaker? If so, which membrane(s) allowed it to pass? Explain the relationship between solute concentration and osmotic pressure. Will osmotic pressure be generated if solutes are able to diffuse? Explain your answer. Because the albumin molecule is too large to pass through a 100 MWCO membrane, you should have noticed the development of osmotic pressure in the left beaker in the albumin run using the 100 MWCO membrane. What do you think would happen to the osmotic pressure if you replaced the deionized water in the right beaker with 9.00 mM albumin in that run? (Both beakers would contain 9.00 mM albumin). What would happen if you doubled the albumin concentration in the left beaker using any membrane? 3-7: In the albumin run using the 200 MWCO membrane, what would happen to the osmotic pressure if you put 10 mM glucose in the right beaker instead of deionized water? Explain your answer. 3-8: What if you used the 100 MWCO membrane in the albumin/glucose run described in the previous question? Title: Explain the relationship between solute concentration and osmotic pressure. Post by: marie03 on Apr 5, 2011 Content hidden
Title: Explain the relationship between solute concentration and osmotic pressure. Post by: alyssa4392 on Apr 11, 2011 Activity 3- Simulating Osmotic Pressure
6. Repeat steps 1 through 5 with the 50, 100, and 200 MWCO membranes. Do you see any evidence of pressure changes in either beaker, using any of the four membranes? If so, which membrane(s)? Na+Cl- and Glucose Does NaCl appear in the right beaker? If so, which membrane(s) allowed it to pass? 50, 100, and 200 8. Explain the relationship between solute concentration and osmotic pressure: As the solute concentration increases, osmotic pressure does also. Will osmotic pressure be generated if solutes are able to diffuse? Explain your answer. No, because you need concentration diffusion. Because the albumin molecule is too large to pass through a 100 MWCO membrane, you should have noticed the development of osmotic pressure in the left beaker in the albumin run using the 100 MWCO membrane. What do you think would happen to the osmotic pressure if you replaced the deionized water in the right beaker with 9.00 mM albumin in that run? (Both beakers would contain 9.00 mM albumin). Nothing would happen. What would happen if you doubled the albumin concentration in the left beaker using any membrane? The pressure would double. In the albumin run using the 200 MWCO membrane, what would happen to the osmotic pressure if you put 10 mM glucose in the right beaker instead of deionized water? Explain your answer. The right beaker's pressure would increase and the left would not do anything. What if you used the 100 MWCO membrane in the albumin/glucose run described in the previous question? It would stay the same. |