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Title: why does the blank have an absorbance reading above zero? Bradford protein assa Post by: kondzia on Jan 12, 2015 Why is it necessary to substract this absorbance from all other results?
Title: Re: why does the blank have an absorbance reading above zero? Bradford protein assa Post by: alyssa_19 on Jan 12, 2015 I am not sure but try if this helps :)
http://www.piercenet.com/method/protein-assay-data-analysis Title: Re: why does the blank have an absorbance reading above zero? Bradford protein assa Post by: rsb on Jan 12, 2015 Quote Why does the blank have an absorbency reading above zero? It should be defaulted to zero. You subtract it to get the difference. Title: Re: why does the blank have an absorbance reading above zero? Bradford protein assa Post by: bio_man on Jan 13, 2015 If it has a reading of zero, that means there is full transmittance, which is what we want, a transparent blank that we can compare our results to.
Title: Re: why does the blank have an absorbance reading above zero? Bradford protein assa Post by: habiba on Jan 13, 2015 Because it absorbs! :) That's why we need to have a blank before we take the absorbance reading of a sample. We don't want to know the absorbance of the sample, not the blank. And we can be sure that we are measuring the absorbance of only the sample by subtracting out the blank's absorbance from the absorbance of your samples. Your spectrophotometer or whatever you are using may do the subtractions automatically for you.
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