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Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Mar 30, 2011 i need help with the answers to the lab physioex exercise 8- chemical and physical processes of digestion Activity 1-5. please
also i have a review sheet with an exercise that asks questions about carbohydrate digestion, protein digestion by pepsin and fat digestion by pancreatic lipase and the action of bile. can anyone please help with the ans..... this lab is so confusing. thanks in advance Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Mar 30, 2011 i got the answers for the review section.....relating to the chemical digestion, protein digestion by pepsin and fat digestion by pancreatic lipase and the action of bile
check this link http://www.hccfl.edu/media/379452/a&p_lab_ex_39b.pdf (http://www.hccfl.edu/media/379452/a&p_lab_ex_39b.pdf) however i am still looking for the answers to the actually activity 2-5 hope this helps anyone interested beautme 25 Title: Chemical and Physical Processes of Digestion Post by: bio_man on Mar 30, 2011 Content hidden
Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Mar 30, 2011 http://wps.aw.com/wps/grader (http://wps.aw.com/wps/grader)
u can get some of the answers here for the activities........ but some of the answers are missing. i hope this helps as well u have to do the online exam and when you submit you will get the answers beautme25 Title: Chemical and Physical Processes of Digestion Post by: bio_man on Mar 30, 2011 http://wps.aw.com/wps/grader (http://wps.aw.com/wps/grader) u can get some of the answers here for the activities........ but some of the answers are missing. i hope this helps as well beautme25 The link doesn't work for me :-\ Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Mar 31, 2011 guysssssssssss help i;m really getting frustrated...... does anyone have the answers for the activity 1-5
beautem25 Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Mar 31, 2011 Re: chemical and physical processes of digestion lab
« Reply #4 on: Today at 10:50 PM » -------------------------------------------------------------------------------- Quote from: beautme25 on Today at 10:47 PM http://wps.aw.com/wps/grader (http://wps.aw.com/wps/grader) u can get some of the answers here for the activities........ but some of the answers are missing. i hope this helps as well beautme25 The link doesn't work for me IN RESPONSE TO THE ABOVE go to any search engine type in "answers to activity 2 chemical and physical processes of digestion" in search box on the page and you will see a link marked Quizzes click on that and you will get the quizz hope it helps beautme25 Title: Chemical and Physical Processes of Digestion Post by: duddy on Mar 31, 2011 Activity 1: Assessing Starch Digestion by Salivary Amylase (pp. 104–105)
10. Tubes 2, 6, and 7 showed the effect of pH on amylase activity. The results of this experiment indicate that the activity maximum of amylase is at pH 7.0, whereas pH 2.0 and pH 9.0 demonstrated very little activity. In this experiment, pH 7.0 showed the highest level of amylase activity. Tube 3 showed that amylase did not contain maltose contamination. Tubes 3, 4, and 5 showed that water had no starch or maltose contamination. Tube 3 directly showed that water did not have maltose or starch contamination. Tube 4 was a starch control (with the same water) that showed no maltose, and tube 5 was a maltose control (also with water) that showed no starch. If control tubes 3, 4, or 5 were not done, then what is perceived as digestion might really be starch or maltose contamination. Saliva would not be active in the stomach because the stomach pH is too low. Boiling inactivates, or denatures, enzymes. Activity 2: Assessing Cellulose Digestion (pp. 105–107) 10. Tubes #4, 5, and 6 showed that starch or cellulose was still present. Tubes #1, 2, 3, and 7 showed positive tests for the Benedict’s reagent, indicating the presence of reducing sugar. Freezing had no effect. Freezing does not restrict enzyme activity, unlike boiling. Amylase had no effect on the cellulose in tube #4. Cellulose is digestible by bacteria. Peptidase had no effect on animal starch. Peptidase does not work on carbohydrate substrates so has no effect on digestion of these molecules. Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Apr 2, 2011 thanks i got some other answers as well and handed in the lab. thanks alot for the help,
beautme25 Title: Chemical and Physical Processes of Digestion Post by: poopler on Apr 24, 2011 Thanks. this lab is really tough and these answers really helped!
Title: Chemical and Physical Processes of Digestion Post by: beautme25 on Apr 24, 2011 np
Re: chemical and physical processes of digestion lab « Reply #9 on: Today at 03:43 PM » Reply with quoteQuote Accept answerMark as Solved Reject answer Thanks. this lab is really tough and these answers really helped! Title: Chemical and Physical Processes of Digestion Post by: quirkycuriousity on Jul 2, 2011 a-s.clayton.edu/.../Chemical%20Processes%20of%20Digestion%20Key.doc (http://a-s.clayton.edu/.../Chemical%20Processes%20of%20Digestion%20Key.doc)
Title: Chemical and Physical Processes of Digestion Post by: sherry17 on Jul 18, 2011 Thanks for the information for the chemical and physical processes of digestion lab. This really help!!! :)
Title: Chemical and Physical Processes of Digestion Post by: bio_man on Jul 18, 2011 a-s.clayton.edu/.../Chemical%20Processes%20of%20Digestion%20Key.doc (http://a-s.clayton.edu/.../Chemical%20Processes%20of%20Digestion%20Key.doc) Thank link doesn't work for me :-\ Title: Chemical and Physical Processes of Digestion Post by: clonline on Jul 24, 2011 Thank you for all the help
Title: Chemical and Physical Processes of Digestion Post by: nurse2be on Aug 8, 2011 I'm getting sooo frustrated with this exercise. I have finally found the answers to the actual activites but can't find anything for the review sheet without buying them.
Title: Chemical and Physical Processes of Digestion Post by: biolove on Aug 9, 2011 I'm getting sooo frustrated with this exercise. I have finally found the answers to the actual activites but can't find anything for the review sheet without buying them. Ask the questions you need help on, what exercise? Title: Chemical and Physical Processes of Digestion Post by: nurse2be on Aug 9, 2011 On Exercise 3. Q: Did the pepsin or deionized water contain and contaminating digested BAPNA? Which tubes confirm this?
Would it be that pepsin did and deionized water did not. Because tubes 2 and 5 did not have an optical density of 0.00? That was my first thought, but then tube 1 DID have and optical density of 0.00 and it had the same substances at tube 2. Title: Chemical and Physical Processes of Digestion Post by: nurse2be on Aug 9, 2011 Also on exercise 4...Q: Can you determine whether fat hydrolysis has occurred in tube 6? Explain your answer.
Would it be yes just because of the fact that the pH after incubation was different than the pH buffer added to the test tube Title: Chemical and Physical Processes of Digestion Post by: how_mendel on Aug 9, 2011 Activity 4: Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile (pp. 109–111)
7. Tube 1 investigated the action of bile on enzyme activity, and tube 2 examined lipase activity without bile. Bile enhances fat digestion by lipase. Yes, you can determine if activity occurred in tube 6 because the pH would drop below pH 9.0. A small amount of fat digestion occurred because pH decreased from 9.0 to 8.97. The optimim pH for lipase activity was pH 7.0. Using a pH method to assay for activity at pH 2.0 does not work because the buffer is already quite acidic. There could have been activity at pH 2.0 that was not detectable by this method. In theory, lipase would be active in the mouth because its pH optimum is relatively neutral. However, it would not be active in the stomach because of the acidic pH condition. The substrate is vegetable oil (fat). The subunit formed is fatty acid (and monoglycerides). Title: Chemical and Physical Processes of Digestion Post by: valgonzalez on Aug 11, 2011 I need help please with exercise 8 activity 3 and 4
Title: Chemical and Physical Processes of Digestion Post by: how_mendel on Aug 11, 2011 I need help please with exercise 8 activity 3 and 4 Start a new topic and I'll help! Title: Chemical and Physical Processes of Digestion Post by: mariahchew on Aug 17, 2011 Having difficulty getting the links to work....any ideas?
Title: Chemical and Physical Processes of Digestion Post by: amber1624 on Sep 1, 2011 When our class did this lab, this is what we were supposed to understand:
Pepsin and acid are more optimal for enzyme action - The acid helped the pepsin act more effeceintly. Boiling the test tubes sped up the process and allowed for a quicker reaction (the Benedict solution began changing color). That change in color was evidence of glucose. Title: Chemical and Physical Processes of Digestion Post by: hgdameron on Oct 12, 2011 I need help with A&P 8.0 Exercise 8 review questions..thx
Title: Chemical and Physical Processes of Digestion Post by: bio_man on Oct 12, 2011 I need help with A&P 8.0 Exercise 8 review questions..thx Could you post the question? Title: Chemical and Physical Processes of Digestion Post by: mariaochoa on Oct 24, 2011 no abrio el enlace que mandaron :(
Title: Chemical and Physical Processes of Digestion Post by: jackiemicheleg on Oct 25, 2011 This thread has helped a bunch, thank you so much! :)
Title: Chemical and Physical Processes of Digestion Post by: p0lo on Nov 1, 2011 Activity 4: Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile (pp. 109–111) 7. Tube 1 investigated the action of bile on enzyme activity, and tube 2 examined lipase activity without bile. Bile enhances fat digestion by lipase. Yes, you can determine if activity occurred in tube 6 because the pH would drop below pH 9.0. A small amount of fat digestion occurred because pH decreased from 9.0 to 8.97. The optimim pH for lipase activity was pH 7.0. Using a pH method to assay for activity at pH 2.0 does not work because the buffer is already quite acidic. There could have been activity at pH 2.0 that was not detectable by this method. In theory, lipase would be active in the mouth because its pH optimum is relatively neutral. However, it would not be active in the stomach because of the acidic pH condition. The substrate is vegetable oil (fat). The subunit formed is fatty acid (and monoglycerides). Thanks, that really helped! Title: Chemical and Physical Processes of Digestion Post by: lhendrix on Mar 10, 2012 thx for your help in this...this was a hard lab :thi:
Title: Chemical and Physical Processes of Digestion Post by: eduk911 on Mar 19, 2012 no work:(
Title: Chemical and Physical Processes of Digestion Post by: samsub91 on Mar 27, 2012 What do tubes 2, 6 and 7 reveal about pH and amylase activity?-pH has a strong affect on amylase activity.
Which pH buffer allowed the highest amylase activity?-7.0 Which tube indicates that the amylase did not contain maltose?-3 Title: Chemical and Physical Processes of Digestion Post by: samsub91 on Mar 27, 2012 4.If control tubes were left out, what objections could be raised against the statement "Amylase digests maltose to starch"?
Contamination of maltose or starch would yield a false positive result. 5.Why would the amylase present in the saliva not be active in the stomach? The pH in the stomach is too low. 6. What effect does boiling have on enzyme activity? Boiling inactivates enzymes. 7. Refer to the data you collected for Activity 2: Assessing Cellulose Digestion. Which tubes showed a positive test for the IKI reagent? Tubes 4, 5, and 6 8.Which tubes showed a positive test for the Benedict's reagent? Tubes 1, 2, 3, and 7 9.What was the effect of freezing tube 1? Freezing had no effect. 10.How does the effect of freezing differ from the effect of boiling? Freezing has no effect on enzyme activity. 11.Which of the following explanations best describes the effect of amylase on glucose in tube 3? Amylase had no effect on the glucose. 12.What was the effect of amylase on cellulose in tube 4? Amylase had no effect on cellulose. 13. Judging from the results of tubes 4, 5, and 7, what can be concluded about the digestion of cellulose? Salivary amylase does not digest cellulose. 14. What was the effect of peptidase in tube 6? Peptidase had no effect because it doesn't work on carbohydrate substrates. 15. Refer to Activity 3: Assessing Protein Digestion by Pepsin. Which pH provided the highest pepsin activity? 2.0 16.Tubes 1 and 2 contained the same substances. Why were their optical density measurements different? The contents of tube 1 were boiled, which inactivated the pepsin; therefore there was no activity in tube 1. 17.What would happen if the temperature were decreased to 10°C? The amount of BAPNA digested would be less. 18. Refer to Activity 4: Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile. What was the difference in activity between test tubes 1 and 2? Tube 1 had greater activity because of the presence of bile. 19 Which pH resulted in maximum lipase activity? 7.0 20. In theory, would lipase be active in the mouth? Yes, in theory lipase would be active in the mouth because the pH optimum is relatively neutral. Title: Chemical and Physical Processes of Digestion Post by: cpeck1207 on Apr 24, 2012 This thread is so helpful with this exercise!
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