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Title: You decide to perform dideoxy sequencing on a PCR product. You add the appropria Post by: mki on Feb 17, 2015 You decide to perform dideoxy sequencing on a PCR product. You add the appropriate 32P-labeled primer, DNA polymerase, DNA template (the PCR product), buffer, dNTP mix, and a small amount of one of the four ddNTPs to four reaction tubes. You run the reactions in the thermal cycler, load each reaction into a separate lane of a polyacrylamide gel, and separate the products by gel electrophoresis. In the figure below, the lanes are labeled according to the ddNTP added.
Title: Re: You decide to perform dideoxy sequencing on a PCR product. You add the appropria Post by: bio_man on Feb 17, 2015 Hello, see if this helps.
Title: Re: You decide to perform dideoxy sequencing on a PCR product. You add the appropria Post by: Bobby Kontonis on Mar 11, 2023 nice
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