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Title: Discuss the similarities and differences between immunodiffusion and immunochrom Post by: Loca on Mar 6, 2015 Discuss the similarities and differences between immunodiffusion and immunochromatographic assays.
Title: Re: Discuss the similarities and differences between immunodiffusion and immunochrom Post by: bio_man on Mar 7, 2015 Hi Loca, while I don't have a specific answer, I happened to find a poster that discusses the effectiveness of the two assays.
Title: Re: Discuss the similarities and differences between immunodiffusion and immunochrom Post by: Loca on Mar 9, 2015 Thank you bio_man!
Title: Re: Discuss the similarities and differences between immunodiffusion and immunochrom Post by: bio_man on Mar 9, 2015 Content hidden
Title: Re: Discuss the similarities and differences between immunodiffusion and immunochrom Post by: Loca on Apr 15, 2015 This is what I came up with... sorry for the delay, I was wicked busy with mid term and papers.
Both assays rely on the diffusion of antibody molecules, and both involve the formation of antibody-antigen complexes. The immunodiffusion assay is a type of precipitation assay in which the presence of antigen-antibody complexes results in the development of a visible line of precipitate where the complexes come out of solution.. It can be used to verify the presence of antibodies reactive to specific antigens. The variation known as radial immunodiffusion can be used to measure antibody concentrations. The procedure requires a high degree of skill and is time-consuming. The immunochromatographic assay detects the presence of antigens rather than antigen-specific antibodies. Antibodies labeled with colored metals form complexes with the antigen, but the complexes do not precipitate out of solution. The soluble, diffusing complexes are trapped and concentrated by anti-antibodies, producing a visible line of color. These immunoassays are very rapid and can be performed by persons with basic clinical skills.. |