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Title: Describe a way that scientists can use to determine if a protein............... Post by: adhungan on Mar 9, 2015 Describe a way that scientists can use to determine if a protein is located on the exoplasmic face of the cell membrane.
Please helppp !! Title: Re: Describe a way that scientists can use to determine if a protein............... Post by: bio_man on Mar 9, 2015 Start with this definitions:
Exoplasmic face You're asking, what characteristics would a peptide have in order to be in the exoplasmic face - I'm not sure :p I did find this information that might help -- Quote Many such proteins contain a single membrane-spanning segment: a sequence of 20 – 25 hydrophobic amino acids that forms a transmembrane α helix, anchoring the protein in the phospholipid bilayer. Most of these single-pass transmembrane proteins have their hydrophilic N-terminal segment on the exoplasmic face and their hydrophilic C-terminal segment on the cytosolic face; other single-pass proteins have the reverse orientation. Many plasma-membrane proteins have multiple membrane-spanning α-helical segments. Such multi- pass transmembrane proteins include the glucose transporter GLUT1 and numerous G protein – linked cell-surface receptors. Still other membrane proteins, which lack a hydrophobic membrane-spanning segment, are linked to a glycosylphosphatidylinositol (GPI) anchor that is embedded in the phospholipid bilayer. Title: Re: Describe a way that scientists can use to determine if a protein............... Post by: adhungan on Mar 9, 2015 Start with this definitions: Exoplasmic face You're asking, what characteristics would a peptide have in order to be in the exoplasmic face - I'm not sure :p I did find this information that might help -- I need the method that scientist use to find where the proteins are located. As in the explasmic face of cell membrane. Quote Many such proteins contain a single membrane-spanning segment: a sequence of 20 – 25 hydrophobic amino acids that forms a transmembrane α helix, anchoring the protein in the phospholipid bilayer. Most of these single-pass transmembrane proteins have their hydrophilic N-terminal segment on the exoplasmic face and their hydrophilic C-terminal segment on the cytosolic face; other single-pass proteins have the reverse orientation. Many plasma-membrane proteins have multiple membrane-spanning α-helical segments. Such multi- pass transmembrane proteins include the glucose transporter GLUT1 and numerous G protein – linked cell-surface receptors. Still other membrane proteins, which lack a hydrophobic membrane-spanning segment, are linked to a glycosylphosphatidylinositol (GPI) anchor that is embedded in the phospholipid bilayer. Title: Re: Describe a way that scientists can use to determine if a protein............... Post by: bio_man on Mar 9, 2015 ???
Title: Re: Describe a way that scientists can use to determine if a protein............... Post by: DrDolittle on Mar 10, 2015 Content hidden
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