|
Title: Why do you pour the ethanol SLOWLY in DNA extraction experiment? Post by: Gardenia on Mar 17, 2015 I am talking about the standard DNA isolation experiment from kiwis and onions.Protocol goes something like this (general and conceptual version follows)
1) Chop the kiwi/onion up finely and add it to a solution of NaCl and detergent 2)Incubate at high temp for a bit 3)Now incubate at cold temp 4)mix well and filter 5)Add the extract to a boiling tube and add neutrase Here's the bit I need explained 6)Slowly trickle x amount of ethanol VERY SLOWLY down the side of the test tube Why do you add it slowly and why do you not add it directly to the solution? Title: Re: Why do you pour the ethanol SLOWLY in DNA extraction experiment? Post by: bio_man on Mar 18, 2015 Why do you add it slowly and why do you not add it directly to the solution? You want a single layer of clear ethanol on top of the mixture. COLD ethanol will work best, as it's the best practice for precipitation. |