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Title: what is the difference between double transformed plasmid and superplasmid Post by: Emma22 on Mar 29, 2015 Hello,
I am having problems distinguishing between superplasmid, double transformed plasmid of a simple recombinant. It is a matter of visualizing the plasmid itself! Title: Re: what is the difference between double transformed plasmid and superplasmid Post by: psyche360 on Mar 30, 2015 Not sure what any of these means lol Don't want a thread to go unanswered!
Explanation of a 'superplasmid' is attached. Double transformations, in which a single cell (typically an Escherichia coli bacterium) is transformed simultaneously with two different plasmids, are deemed by most researchers to be very low frequency events, in particular when both plasmids carry the same antibiotic resistance gene, and/or origin of replication, or when antibiotic selection is applied for one of the plasmids only. Source is below. |