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Title: Micro Help Post by: trin on Jul 15, 2012 What would happen if you forgot to add Gram's Iodine during the staining procedure? Answer Gram positive would stain light purple or give a false G-. Gram negative would stain a light purple or give a false G+ result. Gram positive would stain a darker purple color. Decolorization step would not work. D??? Title: Re: Micro Help Post by: how_mendel on Jul 15, 2012 Iodine (Gram's iodine) solution acts as a mordant and fixes the dye (Crystal violet), making it more difficult to decolorize and reducing some of the variability of the test. So, without it the Crystal violet stain may decolorize. Meaning everything may look red due to the red stain (safranin or carbol fuchsin).
Title: Re: Micro Help Post by: trin on Jul 15, 2012 So D?
Title: Re: Micro Help Post by: how_mendel on Jul 16, 2012 Can't be d, that has to do with alcohol being added, still not 100% sure.
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