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Title: Exercise 10 Post by: jello3211 on Jul 18, 2012 Exercise 10
Questions 1. Discuss the relative convenience of pour- and streak-plate techniques in culturing clinical specimens? 2. Why are plate cultures incubated in the inverted positions? 3. How do you decide which colonies should be picked from a plate culture of a mixed flora? 4. Why is it necessary to make pure subcultures of organisms grown from clinical specimens? 5. How can you determine whether a culture or subculture is pure? 6. What kinds of clinical specimens may yield a mixed flora in bacterial cultures? Page 3 of 5 From Laboratory Manual & Workbook in Microbiology Applications to Patient Care (9th ed.). By Josephine A. Morello, Helen Eckel Mizer, and Paul A. Granato Copyright © 2006 The McGraw- Hill Companies, Inc. Reprinted with permission of The McGraw-Hill Companies, Inc. BIO2071_Microbiology Laboratory 7. When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination? Exercise 16 Questions 1. Define a differential medium and discuss its purpose. 2. Define a selective medium and describe its uses. 3. Why is MacConkey agar selective as well as differential? 4. Why is blood agar useful as a primary isolation medium? 5. How can one distinguish E. coli from P. aerugionosa on Nutrient agar? _____________________________ _____________________________ _ ____________ Blood agar? _____________________________ _____________________________ _ ____________ MAC agar? _____________________________ _____________________________ _ ____________ 6. What is the major difference between Modified Thayer-Martin (MTM) and chocolate agar? When would you use MTM rather than chocolate agar? 7. If you wanted to isolate S. aureus from a pus specimen containing a mixed flora, what medium would you choose to get results most rapidly? Why? 8. What is the value of making a Gram stain directly from a clinical specimen? 9. Why is aseptic technique important in the laboratory? In patient care? Title: Re: Exercise 10 Post by: duddy on Jul 20, 2012 Please show your work, this seems like the whole assignment.
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