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Title: Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results. Post by: Woahscience on May 29, 2015 In class I did a sds-page electrophoresis of e.coli expressed in four different sugars; Frucose, Arabinose, Glucose and Galactose, all in a concentration of 30 µl. When looking at my sds-page result, the four different samples seem to express the same proteins. The only difference that is noticible is the transparency of the blue color, but that's it.
I'm wondering what the reason may be for why this is? Is this suppose to occur, or are different sugars suppose to express different-sized proteins? Sorry for the poor quality of the picture. Note that our results are the ones that can be found above the marker proteins. Please ignore the results below it. Thanks in advance. concentration of 0.5 M sugar* |