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Title: ELISA absorbency question answer checking please Post by: DJC5492 on Aug 25, 2015 In each of the following circumstances indicate whether, for a sandwich assay, you would expect the final absorbance to be increased or decreased.
A) Incomplete coating of well with antigen or antibody - decrease B) Incomplete blocking - increase C) Sample not added - decrease D) Antibody not added - decrease E) Incorrect antibody used - decrease F) Enzyme tag not added - decrease G) Substrate not added - decrease H) Stopping reagent not added - increase I) Incubation temperatures set too high (for example an ELISA validated for incubations at room temperature was run at: a) 37°C and b) 70°C - increase |