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Title: Describe a procedure that you would use to localize the protein dynein in tissue Post by: Islander16 on Nov 23, 2015 describe a procedure that you would use to localize the protein dynein in tissue sections of esophagus and trachea. Indicate expected results
Title: Re: Describe a procedure that you would use to localize the protein dynein in tissue Post by: psyche360 on Nov 23, 2015 You can use immunohistochemistry to stain dynein protein in tissue sections. The cell membranes should be broken by triton.
Counterstain the cells to make them more visible. Use hematox if you are using light microscopy or use DAPI for fluorescence microscopy. Cells with dynein can be identified as coloured complexes. |