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Biology-Related Homework Help Environmental and Conservation Biology Topic started by: tiffany77 on Apr 25, 2016



Title: Could a water sample have a high concentration of the pathogenic bacterium vibri
Post by: tiffany77 on Apr 25, 2016
Could a water sample have a high concentration of the pathogenic bacterium vibrio cholerae and give negative results in the multiple-tube technique? explain


Title: Re: Could a water sample have a high concentration of the pathogenic bacterium vibri
Post by: bio_man on Apr 26, 2016
No it's not possible.

As MPN tests are reliable.MPN test have areputation for producing higly variable results in the numbr of counts but not the wrong results.

The most probable number method, otherwise known as the method of Poisson zeroes, is a method of getting quantitative data on concentrations of discrete items from positive/negative data.The major weakness of MPN methods is the need for large numbers of replicates at the appropriate dilution to narrow the confidence intervals. However, it is a very important method for counts when the appropriate order of magnitude is unknown a priori and sampling is necessarily destructive.