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Title: Enzymes and ice bucket Post by: Retata on May 13, 2016 We store enzymes in -20C.
As I know that it stores with glycerol, which prevent freezing of enzymes. But why we also need to put it on the ice bucket when we are using it? Title: Re: Enzymes and ice bucket Post by: psyche360 on May 16, 2016 Enzymes need to be stored and used at their optimum temperatures. Enzymes are generally stored in glycerol at -20C. This prevents them from freezing completely, which causes protein denaturation and results in a loss of activity. Enzymes will also denature if they are warmed up, repeatedly exposed to temperature fluctuations, or stored at room temperature for extended periods of time. Therefore, enzymes need to be kept cold at all times, even if you are just pipetting out of them quickly.
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