|
Title: What would be the size in base pairs of the largest and smallest amplicons that would be expected if Post by: 05mbodh on May 28, 2016 Relevant details of the STRs are as follows
TH01 Chromosomal location: 11p15.5 (tyrosine hydroxylase, 1st intron) Repeat motif: TCAT (GenBank top strand) although AATG (bottom strand) often used. Note that variants with a [AATG]nATG[AATG]n structure quite common e.g. [AATG]6ATG[AATG]3 would be 9.3 repeats. Allele range: 3-14 For the gene: 1. Identify and mark the introns and exons 2. Identify the repeat region 3. Identify the translation start codon 4. Identify the region where RNA polymerase binds 5. Calculate the number of repeats – does this fit with reported allele ranges? 6. Design suitable “forward” and “reverse” primers for the amplification of the repeat region 7. What would be the size in base pairs of the largest and smallest amplicons that would be expected if those primers were used in PCR. |