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Title: Does a bacteria follow the same growth characteristic when growing in minimal media as in a broth/agar media? Post by: rjandmj82 on Oct 3, 2012 I am supposed to grow my bacteria in minimal media and use for making a microbial fuel cell. The only problem is that i don know how much do i inoculate in my 25% minimal media from a broth culture? what does "use 25% minimal media" mean? does it mean to use 250ml of originally prepared 1L media?
Title: Does a bacteria follow the same growth characteristic when growing in minimal media as in a broth/agar media? Post by: rja5200 on Oct 3, 2012 yep to inoculate you just take a few colonies from the agar plate and put it in your 25% minimal media. Usually we use a tooth pick to scrape a few colonies, and drop it in, you dont need many.
The 'use 25% minimal media' is exactly how you said. Dilute the minimal media to 25%. So if you wanted 1L total, 750mL of distalled water and 250mL of minimal media. Be careful to check its not already diluted for you, often it is. have fun |