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Title: Why do heat and extremes of pH cause unfolding? Post by: Starfire11 on Sep 25, 2016 Why do heat and extremes of pH cause unfolding?
Select all that apply a. Extremes of pH provide high concentration of either acid or base that causes the hydrolysis of disulfide bonds. b. Heating adds energy to the system, making it more difficult for the noncovalent bonds to maintain the polypeptide in its native conformation. c. Heating adds energy to the system, thereby disrupting both primary and secondary structures of protein. d. Extremes of pH cause polarization of the protein molecule, thereby disrupting hydrophobic interactions. e. Extremes of pH cause changes in the ionization or protonation state of some functional groups, thereby disrupting both ionic and hydrogen bonds. Title: Re: Why do heat and extremes of pH cause unfolding? Post by: bio_man on Feb 20, 2018 Here's what I got:
Answer is 1. Extremes of pH cause changes in the ionization or protonation state of some functional groups thereby disrupting both ionic and hydrogen bonds. Protonation of the amino acid residues due to decrease in the pH/ acidic pH decides whether or not it will participate in hydrogen bonding and thus an alteration in pH can denature a protein. Answer is 2. Heating adds energy to the system making it more difficult for the no covalent bonds to maintain the polypeptide in its native conformation. The quarternary structure of a multimeric protein is stabilised by hydrogen bonding, ionic bonding, hydrophobic interactions. These non-covalent interactions are also present in the tertiary structure of monomeric proteins. These non-covalent interactions become unstable due to increase in the heat of the system and thus cannot maintain the quarternary or tertiary structure of the protein which leads to its denaturation. Title: Re: Why do heat and extremes of pH cause unfolding? Post by: psyche360 on Feb 20, 2018 Great answer
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