Biology Forums - Study Force

Looking for help with the questions from exercise 12 activities 1 and 3 in 8.0.  These labs ARE NOT in the manual.  There are online through the cd online.


Activity #1
1.)Which patients have chlamydia?
2.) Are we testing for antigens or antibodies?
3.) Did the controls give the expected results? Explain
4.) Describe the significance of the washing step.


Activity #3
1.) Which patient is positive for HIV?
2.) Are we testing for antigens or antibodies?
3.) What key substance is present in "Developing Buffer"?
4.) What does seroconversion mean? Which patient would be positive for seroconversion?

Thank you!!!!

Does this help :down:

1. The antigen is found on the patient sample. Since the test is a direct test, it detects antigen. The antigens detected are the elementary bodies.

2. The antigen is the entire foreign particle whereas the epitope is the specific binding site on the antigen that antibodies bind to. Antigens can have multiple epitopes with different structures.

3. Patient sample C displayed some nonspecific binding that was not removed by the washing steps.

Answers 1 & 3 were helpful and of course the chart is always helpful to compare results with.

Anything for activity 3??

1. In this indirect ELISA we are testing for the presence of antibodies in the patient sample. The antibodies are a result of the presence of antigen.
2. The secondary antibody binds to the constant region of the primary antibody. It is specifically prepared to do this so that it can detect the presence of the primary antibody in the patient sample.
3. Seroconversion is when a patient sample contains antibodies against a particular antigen. We say the serum has converted from not containing the antibody to a condition where it does contain the antibody.