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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
Post by: monroe025 on Nov 13, 2012
PhysioEx 9.0 Ex. 8: Chemical and Physical
Processes of Digestion - Review Sheet

 Assessing Starch Digestion by Salivary Amylase
1. List the substrate and the subunit product of amylase. _starch & maltose respectively__

2. What effect did boiling and freezing have on enzyme activity? Why? How well did the results compare with your prediction?
__Boiling caused amylase to be denatured, thus inactivating the enzyme. Freezing has no effect. The function of an enzyme is directly related to its environment, like temperature.

3. At what pH was the amylase most active? Describe the significance of this result.  pH  7.0. Amylase is most active in neutral areas, such as the mouth and the small intestine (duodenum)._

4. Briefly describe the need for controls and give an example used in this activity. _Controls are needed to validate the results of the experiment. One example is testing for sugar using Benedict's test on Amylase The negative control was used to detect if amylase was contaminated with maltose or not._

5. Describe the significance of using a 37°C incubation temperature to test salivary amylase activity. __37°C simulates an environment of normal body temperature. ___
A C T I V I T Y 2
 Exploring Amylase Substrate Specificity
1. Describe why the results in tube 1 and tube 2 are the same. _In tube #1 the amylase is hydrolyzing the starch to glucose, & in tube #2 the glucose is already present in the hydrolyzed form.__

2. Describe the result in tube 3. How well did the results compare with your prediction? _ Amylase cannot digest cellulose, so tube #3 is not positive for Benedict’s test.__

3. Describe the usual substrate for peptidase.  _A protein (BAPNA in this experiment).__

4. Explain how bacteria can aid in digestion. _Bacteria can aid in digestion by breaking down cellulose with the enzyme cellulase, humans do not produce this enzyme.
A C T I V I T Y 3
 Assessing Pepsin Digestion of Protein
1. Describe the effect that boiling had on pepsin and how you could tell that it had that effect. _Boiling denatured the enzyme. You can tell because the protein was not digested in tube #1. There was no color change & a density of 0.__

2. Was your prediction correct about the optimal pH for pepsin activity? Discuss the physiological correlation behind your results.
_Yes. The optimum pH matches the pH secreted by gastric glands. Gastric juice is also close to pH 2.___

3. What do you think would happen if you reduced the incubation time to 30 minutes for tube 5? _This would reduce digestion in tube 5.__
A C T I V I T Y 4
Assessing Lipase Digestion of Fat
1. Explain why you can’t fully test the lipase activity in tube 5. _Measurement of lipase activity uses a decrease in pH. Because the pH in Tube #5 is already very low, it is hard to tell if fatty acids are released.__

2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible reasons why it may or may not have matched. __Test tube #1 should have the highest activity because the pH is closest to that of the small intestine.__

3. Explain why pancreatic lipase would be active in both the mouth and the intestine. _Pancreatic lipase is most active at pH 7.0 The pH of the mouth is 7.0 & the pH of the small intestine is close to 8.0 so the enzyme would function in both places.___

4. Describe the process of bile emulsification of lipids and how it improves lipase activity. _Fat globules are separated into droplets by bile salts through an emulsification process which is physical not chemical, which promote lipase activity__


Hope this helps someone!


Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
Post by: scornell1118 on Mar 25, 2013
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
Post by: monroe025 on Oct 15, 2013
Glad this is still helping people!


Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
Post by: Evelyn Sumo on Apr 14, 2016
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Title: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
Post by: HelpINeedSomebo on Jun 2, 2016
I have just decided in the 11th hour to change my major from nursing to biotechnology and am so glad to see someone has put the Physio-Ex Labs up. I have a CD Rom of physio-ex 11 (Marieb A&P), ? Lab Pal 8.0...perhaps and a complete histology atlas...but I still haven't located it.  I just re-enrolled with the site this morning in the wee hours and have not completely figured out where and how to post study materials.  I remember the Physio-Ex sessions as being life-savers for me however and this one in particular as you are studying digestion and such at the end of A&P II...these sim labs really made sense when we were unable to perform the actual lab.

Sorry....rambled too long...too much coffee too early.


Title: Re: PhysioEx 9.0 Ex 8 Answers - Chemical and physical digestion
Post by: Kallas on Sep 16, 2016
Thanks for the help