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Title: Determine whether a specific DNA fragment in yeast is present in wild type versus mutant DNA .. ... Post by: ehd123 on Dec 18, 2016 To determine whether a specific DNA fragment in yeast is present in wild type versus mutant DNA .. which technique should I use?
a)digest genomic dna with restriction enzymes and run on gel and compare band sizes b)perform southern blot c)perform western blot d) perform northern blot e)perform reverse transcription-PCR f)a and b g) a and d Title: Re: Determine whether a specific DNA fragment in yeast is present in wild type versus mutant DNA .. ... Post by: akorne on Feb 16, 2017 a and b (technique a is necessary for technique b).
c) is a procedure specifically for proteins so is not applicable. d) is a procedure specifically for RNA so is not applicable. e) might be possible if the mutant piece of DNA is long enough for a PCR. If it was small e.g. 40 bases then PCR would be impossible. Title: Re: Determine whether a specific DNA fragment in yeast is present in wild type versus mutant DNA .. ... Post by: ehd123 on Feb 25, 2017 Thank you for your input!
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