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Laboratory Help Upper-Year Courses Topic started by: smm0309 on Dec 23, 2012



Title: Can nucleic acids be detected by the ELISA format?
Post by: smm0309 on Dec 23, 2012
I was wondering if ELISA could detect nucleic acids and If so why? and if not, why not?


Title: Can nucleic acids be detected by the ELISA format?
Post by: rizzlepuff8 on Dec 23, 2012
i would have to say no... The ELISA format by definition is used to detect antigens with antibodies. So since there's no antibodies for DNA or RNA, you cannot do ELISA. Perhaps a similar method is chip on chip, where you immunoprecipitate the DNA with antibodies detecting transcription factors or activators etc. And then bind those DNA to a microarray


Title: Can nucleic acids be detected by the ELISA format?
Post by: rivers.butterfly on Dec 23, 2012
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