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Title: How to prevent telomeres from shortening? Post by: BioRumor on Aug 30, 2010 My ^*%&^%$& telomeres keep getting shorter and shorter, no matter what i try to do.
I tried injecting a little telomerase reverse transcriptase into each of my cells, but that approach just seems to take FOREVER... Any ideas? (this was just a test posting, but if you happen to know the solution, I'd be pretty darn happy!) Title: How to prevent telomeres from shortening? Post by: bio_man on Aug 30, 2010 Hey BioRumor, thanks for joining :)
To my understanding, telomeres are segment of DNA that occurs at the ends of chromosomes. I remembered that from 2nd year cell biology. Similarly, after every cellular replication, telomeres shorten, but to prevent damage, we have telomerases to increase their length. They can do this because telomerases have an RNA copy of their own to create repeating units of more DNA. If you're doing thing in vitro make sure your have the correct temperature settings and right amount of enzymes. Moreover, doesn't it also depend on the RNA sequence found on the telomerase enzyme itself, perhaps it doesn't correspond to the DNA sequence? ??? Title: How to prevent telomeres from shortening? Post by: duddy on Aug 30, 2010 Moreover, doesn't it also depend on the RNA sequence found on the telomerase enzyme itself, perhaps it doesn't correspond to the DNA sequence? ??? bio_man, telomerase is a widely conserved enzyme so I don't think that would be the case BioRumor, this may be of some help to you, read the attachment (I highlighted some key areas for you) Concise Review: Telomere Biology in Normal and Leukemic Hematopoietic Stem Cells Title: How to prevent telomeres from shortening? Post by: Biology Forums on Sep 4, 2010 So what ended up happening with your experiment?
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