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Title: Why dilute ligation mixture before transformation? Post by: adriata on Mar 15, 2017 I understand that diluting the ligation reaction mixture prior to transformation increases the transformation efficiency, but why is it that less DNA yields better results in this case?
Title: Re: Why dilute ligation mixture before transformation? Post by: 5seal on Mar 16, 2017 Content hidden
Title: Re: Why dilute ligation mixture before transformation? Post by: psyche360 on Mar 16, 2017 At high [DNA] you also could have an increased amount of 'impurities' (salts, etc) that can affect transformation efficiencies.
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